Sealed item rate tenders in two part bidding systems (Part –I-technolo-commercial and Part-II-price bid) are invited from bonafied, resourceful and eligible manufacturers/exclusive distributors for “ Isothermal Titration Calorimeter” at Indian Association for the Cultivation of Science, 2A & 2B, Raja S. C. Mullick Road, Kolkata -700032 by 3.00 pm. of 27.10.2010. 

Specifications for Isothermal Titration Calorimeter

The system must be complete with control unit, degassing station, filling & cleaning assemblies, injection syringes, other start-up accessories, necessary software/s for instrument control, operation analysis of data, viewing and printing enabling accurate determination of thermodynamic parameters such as binding constants, reaction stoichimetry, enthalpy, entropy etc.  with the following detailed specifications: 

Operating temperature range: 2 ^o C to 80 ^o C 

Temperature stability ± 0.005 ^o C measured at different time periods 

Sensitivity: High sensitivity with base line noise level around 0.5 nCal / sec with a 2 second data filter for ultra sensitive studies 

Minimum Response Time (high gain):  20 seconds 

Minimum detectable heat effect 0.1 μJ 

Mixing speed and Response: Variable mixing speed and variable response times 

Binding affinity: The unit must have the capability to directly measure binding constants accurately in the range of 10² to 10⁹ M^-1 and tight binding constants by displacement reactions in the order higher than 10⁹ M^-1 

Heat compensation to cells should be via power feedback only for accurate, sensitive heat determination and rapid response. No solid state heating and cooling systems. 

Cell type: Non-capillary, fixed in place – non-removable, with low working sample volume of 1.5 ml or lower.  They should be enclosed in an adiabatic chamber

Cell design: Coin-shape - providing large surface area for the peltier elements and makes it easier to attach them. Perfect Cell Geometry (coin shaped cell). Fast Equilibration Time. Fast Response Time.

Cell Material: should be made up of Hastelloy material which is very inert. No reaction with S-containing groups or reducing agents. Should have Excellent Chemical resistance. Should work at pH range 2 – 12 of solvents. 

The System should have three user selectable modes of operation (Response Times) high gain, low gain and medium gain - allowing the fastest re-equilibration between injections, there by providing the shortest experimental times.   

There should be no use of external water bath etc.  The device should be internal (peltier type). 

The system should have ThermoVac for degassing. 

Required Computer to form an integral part of the supply. 

The software should be Origin®, capable of providing user selectable binding models and data merging like: • Single Site • Two Site • Sequential Site • Competitive Site • Enzyme Kinetics

• Displacement • Dissociation.     

For multiple run analysis.

Instrument operation manuals (English language) should be included in the standard scope.   

Trained technicians should install the instrument and ensure it is operating correctly and within specifications.  User training at site should also be provided. 

Users lists of similar equipment supplied in India should be provided (Enclose full list of users in India). Vendor should have couple of users available in last 5 years in the eastern zone of India. A detailed technical compliance is required with supporting data where possible to show compliance

Registrar